LOCUS       pSK-GFP        4095 bp    DNA     circular SYN 14-FEB-2022
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Demo User
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Sep 29, 2021 from SnapGene Viewer 5.1.7 to 
            Vector NTI(R) format
            https://www.snapgene.com
COMMENT     Storage Type: Basic
            Original Author:
            Demo User
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-14264408|
COMMENT     VNTDBDATE|-14264408|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pSK-GFP|
COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     misc_feature    complement(263..569)
                     /vntifkey="21"
                     /label=f1
     misc_feature    754..772
                     /vntifkey="21"
                     /label=T7\promoter
     misc_feature    771..823
                     /vntifkey="21"
                     /label=5'\\UTR
     primer          794..823
                     /vntifkey="27"
                     /label=PBNT5end
                     /note="sequence: TCCCCACAGACTCAGAGAGAACCCGCCACC"
     primer          complement(794..823)
                     /vntifkey="27"
                     /label=MBNT5end
                     /note="sequence: GGTGGCGGGTTCTCTCTGAGTCTGTGGGGA"
     primer          1550..1579
                     /vntifkey="27"
                     /label=PBNT3end
                     /note="sequence: GAGCTGGTACTGCATGCACGCAATGCTAGC"
     primer          complement(1550..1579)
                     /vntifkey="27"
                     /label=MBNT3end
                     /note="sequence: GCTAGCATTGCGTGCATGCAGTACCAGCTC"
     misc_feature    1553..1835
                     /vntifkey="21"
                     /label=3'\UTR
     misc_feature    1842..1952
                     /vntifkey="21"
                     /label=polyA
     primer          complement(2034..2056)
                     /vntifkey="27"
                     /label=T3P
                     /note="sequence: cgcaattaaccctcactaaaggg"
     misc_feature    complement(2034..2053)
                     /vntifkey="21"
                     /label=T3\P
     primer          complement(2358..2379)
                     /vntifkey="27"
                     /label=PT6265\seq
                     /note="sequence: cgtattaccgcctttgagtgag"
     primer          2412..2435
                     /vntifkey="27"
                     /label=P-DL-1
                     /note="sequence: agaacatgtgagcaaaaggccagc"
     misc_feature    complement(2420..3087)
                     /vntifkey="21"
                     /label=pUC\ori
     primer          complement(3091..3112)
                     /vntifkey="27"
                     /label=M-Pica-3561
                     /note="sequence: ttcgttccactgagcgtcagac"
     misc_feature    complement(3238..4095)
                     /vntifkey="21"
                     /label=Ampicillin
     misc_feature    824..1540
                     /vntifkey="21"
                     /label=eGFP
BASE COUNT     1101 a      1094 c      1011 g       889 t 
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa 
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa 
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac 
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa 
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct 
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa 
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc 
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc 
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 
      721 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata ggaataaact 
      781 agtattcttc tggtccccac agactcagag agaacccgcc accatggtga gcaagggcga 
      841 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca 
      901 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa 
      961 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac 
     1021 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa 
     1081 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa 
     1141 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct 
     1201 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta 
     1261 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt 
     1321 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa 
     1381 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc 
     1441 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac 
     1501 cgccgccggg atcactctcg gcatggacga gctgtacaag tgatgactcg agctggtact 
     1561 gcatgcacgc aatgctagct gcccctttcc cgtcctgggt accccgagtc tcccccgacc 
     1621 tcgggtccca ggtatgctcc cacctccacc tgccccactc accacctctg ctagttccag 
     1681 acacctccca agcacgcagc aatgcagctc aaaacgctta gcctagccac acccccacgg 
     1741 gaaacagcag tgattaacct ttagcaataa acgaaagttt aactaagcta tactaacccc 
     1801 agggttggtc aatttcgtgc cagccacacc ctggagctag caaaaaaaaa aaaaaaaaaa 
     1861 aaaaaaaaaa agcatatgac taaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 
     1921 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagcttgata tcgaattcct gcagcccggg 
     1981 ggatccacta gttctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta 
     2041 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 
     2101 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 
     2161 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 
     2221 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 
     2281 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 
     2341 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 
     2401 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 
     2461 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 
     2521 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 
     2581 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 
     2641 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 
     2701 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 
     2761 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 
     2821 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 
     2881 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 
     2941 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 
     3001 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 
     3061 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 
     3121 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 
     3181 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 
     3241 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 
     3301 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 
     3361 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 
     3421 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 
     3481 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 
     3541 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 
     3601 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 
     3661 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 
     3721 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 
     3781 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 
     3841 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 
     3901 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 
     3961 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 
     4021 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 
     4081 atgttgaata ctcat 
//
